Detailed view for Tb927.7.1450

Basic information

TDR Targets ID: 16701
Trypanosoma brucei, Conserved hypothetical ATP binding protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.7208 | Length (AA): 321 | MW (Da): 36382 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03029   Conserved hypothetical ATP binding protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0000166   nucleotide binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 205 5hcn (A) 6 185 32.00 0.000000000025 1 0.872868 0.04
8 223 1yr7 (A) 7 188 35.00 0.00000058 1 0.858597 0.35

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128188)

Species Accession Gene Product
Arabidopsis thaliana AT5G22370   early embryo development protein QQT1
Babesia bovis BBOV_IV003790   ATP binding family protein
Babesia bovis BBOV_IV003840   ATP binding family protein, putative
Brugia malayi Bm1_07855   Conserved hypothetical ATP binding protein
Candida albicans CaO19.10678   similar to S. cerevisiae YOR262W
Candida albicans CaO19.3169   similar to S. cerevisiae YOR262W
Caenorhabditis elegans CELE_B0207.6   Protein B0207.6
Cryptosporidium hominis Chro.70020   hypothetical protein
Cryptosporidium parvum cgd7_80   XPA1 binding protein-like GTpase
Dictyostelium discoideum DDB_G0281787   GPN-loop GTPase 2
Drosophila melanogaster Dmel_CG10222   CG10222 gene product from transcript CG10222-RB
Echinococcus granulosus EgrG_000894700   GPN loop GTPase 2
Entamoeba histolytica EHI_108650   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000894700   GPN loop GTPase 2
Giardia lamblia GL50803_14109   ATP-binding protein
Homo sapiens 54707   GPN-loop GTPase 2
Leishmania braziliensis LbrM.26.0500   hypothetical protein, conserved
Leishmania donovani LdBPK_260470.1   hypothetical protein, conserved
Leishmania infantum LinJ.26.0470   hypothetical protein, conserved
Leishmania major LmjF.26.0480   hypothetical protein, conserved
Leishmania mexicana LmxM.26.0480   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_08306   hypothetical protein
Loa Loa (eye worm) LOAG_02653   hypothetical protein
Mus musculus ENSMUSG00000028848   GPN-loop GTPase 2
Neospora caninum NCLIV_052100   hypothetical protein
Oryza sativa 4329653   Os02g0555000
Plasmodium berghei PBANKA_0818700   GPN-loop GTPase, putative
Plasmodium falciparum PF3D7_0917700   GPN-loop GTPase, putative
Plasmodium knowlesi PKNH_0715700   GPN-loop GTPase, putative
Plasmodium vivax PVX_099305   XPA binding protein 1, putative
Plasmodium yoelii PY06079   similar to unknown protein
Saccharomyces cerevisiae YOR262W   Gpn2p
Schistosoma japonicum Sjp_0000530   ko:K06883 ATP binding domain 1 family, member B, putative
Schistosoma mansoni Smp_165330   xpa-binding protein 1-related
Schmidtea mediterranea mk4.000466.02  
Trypanosoma brucei gambiense Tbg972.7.1450   hypothetical protein, conserved
Trypanosoma brucei Tb927.7.1450   Conserved hypothetical ATP binding protein, putative
Trypanosoma cruzi TcCLB.503579.40   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.507517.30   hypothetical protein, conserved
Toxoplasma gondii TGME49_215090   ATP binding protein, putative
Theileria parva TP01_1015   hypothetical protein, conserved
Trichomonas vaginalis TVAG_208450   xpa-binding protein, putative

Essentiality

Tb927.7.1450 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.1450 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.1450 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.1450 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.1450 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_B0207.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_B0207.6 Caenorhabditis elegans slow growth wormbase
YOR262W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0818700 Plasmodium berghei Essential plasmo
TGME49_215090 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.7.1450 (Trypanosoma brucei), Conserved hypothetical ATP binding protein, putative
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